
What is Test Tube?
A test tube is a small, cylindrical glass or plastic container with a rounded bottom and an open top. They come in different sizes, but most are 10 to 20 cm long and 1 to 2 cm wide—just the right size for holding small amounts of substances in the lab!
Important Features

- Made of Heat-Resistant Glass – Most test tubes are made of borosilicate glass or fused quartz, which can handle high temperatures without breaking.
- Flared Lip – The open top usually has a slightly flared edge to make pouring liquids easier and more controlled.
- Different Bottom Shapes – While most test tubes have a rounded bottom, some have flat or conical bottoms for specific uses. The rounded bottom and straight sides help prevent spills, make the test tube easy to wash, and allow scientists to see reactions clearly.
- Narrow Necks – The long, thin neck slows down the release of gases, making it useful for handling substances that evaporate quickly.
- Some Feature Sealable Designs – Some test tubes come with ground glass stoppers or screw caps to keep chemicals secure.
- Labeling Area – Many test tubes have a white or frosted section near the top where scientists can write labels with a pencil for easy identification.
Uses
Test tubes are commonly used in chemistry and other scientific fields for various purposes, including:
- Chemical Reactions – Conducting small-scale reactions, such as mixing reactants to observe color changes, gas production, or precipitate formation.
- Heating Substances – Heating liquids or solids over a Bunsen burner or water bath, often with a test tube holder.
- Sample Storage – Holding small liquid or solid samples for later analysis or testing.
- Culturing Microorganisms – Growing bacteria, fungi, or other microorganisms in liquid or solid media.
- Centrifugation – Holding samples for separation of components based on density using a centrifuge.
- Distillation and Evaporation – Used to observe phase changes or remove solvents by gentle heating.
- Gas Collection – Collecting and analyzing gases produced in chemical reactions.
Types

There are several types of test tubes, each designed for specific laboratory applications. Here are some of the most common types:
- Standard Test Tubes: These are the most common types of test tubes. Basic glass or plastic tubes used for general chemical reactions and sample storage.
- Boiling Tubes: Larger and thicker-walled than standard test tubes, designed for heating substances over an open flame.
- Ignition Tubes: Small, heat-resistant tubes used for high-temperature reactions, such as combustion experiments.
- Culture Tubes: Used in microbiology for growing bacteria, fungi, or cell cultures; may have screw caps or cotton plugs to prevent contamination.
- Centrifuge Tubes: Made to withstand high-speed rotation in a centrifuge, often featuring a conical or tapered bottom for efficient separation of substances.
- Blood Collection Tubes (Vacutainers): Used in medical settings to collect blood samples, often pre-filled with anticoagulants or clot activators.
- NMR Tubes (Nuclear Magnetic Resonance Tubes): Thin-walled tubes made of specialized glass for use in NMR spectroscopy to study molecular structures.
- Test Tubes with Stoppers: Equipped with ground glass stoppers, screw caps, or rubber stoppers to seal and store volatile or reactive substances.